
vitrification
#1
Posted 03 January 2011 - 11:41 AM
The long testing method that takes 2 weeks for results requires vitrification.
I believe you previously replied that the thaw rate for vitrified embryos was about 80% which is what my clinic stated as well.
But I'm confused as US clinics stating thaw rates from vitrification are upwards of 95% (they also say that the attrition rate for the CGh normal embryos is negligiable). Can you help me to understand the reported differences in thaw rates, as this is of concern to me.
The US clinics doing the longer form of CGH analysis comment that micro array CGH is not able to get precise enough info from a single cell. That said, having come across this article on Reprogenetic's website, http://reprogenetics.com/news/news_17.pdf,I'm confused. To a non scientific eye, this looks like aCGH is reliable and ready to go.
Can you offer any clarifications? Is there a difference in risk between polar body biopsy and day 3 biopsy?
I'm now sitting on my 2nd pregnancy which is headed towards miscarriage, and struggling to know if I have any good options to prevent this from happenign again, aside from Donor Egg.
Your clarifications would be appreciated more than you know.
Thanks,
A.N.
TTC from March 2009-March 2011 before DB had arrived on the scene
About 9 IUIs, 3 IVFs total
2 pregnancies with 7 week miscarriage requiring D&C
2 chemical pregnancies
April 2011 began a relationship now with a wonderful guy with an 8 year old son.
Sept 2011 Natural BFP while trying not to conceive (how ironic)
First Beta Day 15 from LMP was 851, Beta 4, 19 days post ovulation 5382
7wk US showed a heartbeat of 150! 9 wk US HB 185
May 19 2012 DS is born!!
#2
Posted 23 January 2011 - 11:38 AM
I'm interested in the new methods of PGD allowing all chromosomes to be tested.
The long testing method that takes 2 weeks for results requires vitrification.
I believe you previously replied that the thaw rate for vitrified embryos was about 80% which is what my clinic stated as well.
But I'm confused as US clinics stating thaw rates from vitrification are upwards of 95% (they also say that the attrition rate for the CGh normal embryos is negligiable). Can you help me to understand the reported differences in thaw rates, as this is of concern to me.
The US clinics doing the longer form of CGH analysis comment that micro array CGH is not able to get precise enough info from a single cell. That said, having come across this article on Reprogenetic's website, http://reprogenetics.com/news/news_17.pdf,I'm confused. To a non scientific eye, this looks like aCGH is reliable and ready to go.
Can you offer any clarifications? Is there a difference in risk between polar body biopsy and day 3 biopsy?
I'm now sitting on my 2nd pregnancy which is headed towards miscarriage, and struggling to know if I have any good options to prevent this from happenign again, aside from Donor Egg.
Your clarifications would be appreciated more than you know.
Thanks,
A.N.
ICSI#1 LP 2 eggs/2 fertilised 1x4 cell, 1x2 cell on 2DT BFN
ICSI#2 SP 6 eggs/6 fertilised 2x8 cells on 3DT BFN
FET 2x8 cells BFN
Moved to DE
DEICSI #1 8 mature eggs but only 1 fertilised. 1x9 cell on 3DT....BFP!!!!
HB @ 6+4, no HB @ 8+4 MMC, D&C...totally heartbroken :,(
ICSI#3 (back to my own eggs) LP (error by consultant) 2 eggs but only 1 mature but it fertilised. 11 cells @ 3DT. BFN
DEICSI #2 April 2011
#3
Posted 23 January 2011 - 11:44 AM
The embryos would then be put back on a FET cycle the following month ensuring that only chromosomally normal eggs were replaced. Only 2 eggs at a time would be put back. The rate of pregnancy for over 40 rose from 7-9% to 65% so equal to DE really. Sadly we only got 2 eggs and only 1 was mature so it was considered pointless to spend the money on aCGH.
We did a DE cycle and I suffered a MMC at 8+4 so the chance of MC is still there with DE. Good luck xxx
ICSI#1 LP 2 eggs/2 fertilised 1x4 cell, 1x2 cell on 2DT BFN
ICSI#2 SP 6 eggs/6 fertilised 2x8 cells on 3DT BFN
FET 2x8 cells BFN
Moved to DE
DEICSI #1 8 mature eggs but only 1 fertilised. 1x9 cell on 3DT....BFP!!!!
HB @ 6+4, no HB @ 8+4 MMC, D&C...totally heartbroken :,(
ICSI#3 (back to my own eggs) LP (error by consultant) 2 eggs but only 1 mature but it fertilised. 11 cells @ 3DT. BFN
DEICSI #2 April 2011